Initiation of DNA synthesis by microtubule disruption in quiescent rat 3Y1 cells
نویسندگان
چکیده
منابع مشابه
Initiation of DNA synthesis by nuclei from scrape-ruptured quiescent mammalian cells in high-speed supernatants of Xenopus egg extracts.
Demembranated sperm heads, detergent-isolated somatic nuclei and even naked DNA are efficiently replicated in cytoplasmic extracts of activated amphibian eggs, but only after nuclear assembly and the formation of an intact nuclear envelope. DNA synthesis has not previously been shown to be initiated in high-speed (200,000 g) supernatants of egg cytoplasm because they are depleted of the vesicul...
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Suspension cultures of Chinese hamster cells (line CHO) were grown to stationary phase (approximately 8-9 x 10(5) cells/ml) in F-10 medium. Cells remained viable (95%) for at least 80 hr in stationary phase, and essentially all of the cells were in G(1) Upon resuspension or dilution with fresh medium, the cells were induced to resume traverse of the life cycle in in synchrony, and the patterns ...
متن کاملInitiation of DNA replication in nuclei from quiescent cells requires permeabilization of the nuclear membrane
We have investigated the replication capacity of intact nuclei from quiescent cells using Xenopus egg extract. Nuclei, with intact nuclear membranes, were isolated from both exponentially growing and contact-inhibited BALB/c 3T3 fibroblasts by treatment of the cells with streptolysin-O. Flow cytometry showed that > 90% of all contact-inhibited cells and approximately 50% of the exponential cell...
متن کاملInitiation of DNA synthesis in cell cultures by colcemid.
Mitotic inhibitors (colcemid, colchicine, and vinblastine) initiate DNA synthesis in dense stationary cultures of mouse embryo fibroblast-like cells. This initiation is not due to any changes in local cell population density. Relationships between the activation of proliferation and other changes of interphase fibroblast-like cells produced by the mitotic inhibitors (disappearance of cytoplasmi...
متن کاملMicrotubule disruption modulates Ca(2+) signaling in rat cardiac myocytes.
Microtubules have been shown to alter contraction in cardiac myocytes through changes in cellular stiffness. However, an effect on excitation-contraction coupling has not been examined. Here we analyze the effects of microtubule disruption by 1 micromol/L colchicine on calcium currents (I(Ca)) and [Ca(2+)](i) transients in rat ventricular myocytes. I(Ca) was studied using the whole-cell patch-c...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1989
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1989.tb14924.x